High prevalence of scrapie in a dairy goat herd: tissue distribution of disease-associated PrP and effect of PRNP genotype and age

Titre du document / Document title

High prevalence of scrapie in a dairy goat herd: tissue distribution of disease-associated PrP and effect of PRNP genotype and age

Auteur(s) / Author(s) GONZALEZ Lorenzo (1) ; MARTIN Stuart (1) ; SISO Silvia (1) ; KONOLD Timm (2) ; ORTIZ-PELKEZ Angel (2) ; PHELAN Laura (2) ; GOLDMANN Wilfred (3) ; STEWART Paula (3) ; SAUNDERS Ginny (2) ; WINDL Otto (2) ; JEFFREY Martin (1) ; HAWKINS Stephen A. C. (2) ; DAWSON Michael (2) ; HOPE James (1) ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s) (1) Veterinary Laboratories Agency (VLA-Lasswade), Pentlands Science Park, Penicuik, Midlothian EH26 OPZ, ROYAUME-UNI (2) VLA-Weybridge, Addlestone, Surrey KT15 3NB, ROYAUME-UNI (3) Roslin Institute Neuropathogenesis Division, Roslin, Midlothian EH25 9PS, ROYAUME-UNI

Résumé / Abstract

Following a severe outbreak of clinical scrapie in 2006-2007, a large dairy goat herd was culled and 200 animals were selected for post-mortem examinations in order to ascertain the prevalence of infection, the effect of age, breed and PRNP genotype on the susceptibility to scrapie, the tissue distribution of disease-associated PrP (PrPd), and the comparative efficiency of different diagnostic methods. As determined by immunohistochemical (IHC) examinations with Bar224 PrP antibody, the prevalence of preclinical infection was very high (72/200; 36.0%), with most infected animals being positive for Prpd in lymphoreticular system (LRS) tissues (68/72; 94.4%) compared to those that were positive in brain samples (38/72; 52.8%). The retropharyngeal lymph node and the palatine tonsil showed the highest frequency of PrPd accumulation (87.3% and 84.5%, respectively), while the recto-anal mucosa-associated lymphoid tissue (RAMALT) was positive in only 30 (41.7%) of the infected goats. However, the efficiency of rectal and palatine tonsil biopsies taken shortly before necropsy was similar. The probability of brain and RAMALT being positive directly correlated with the spread of PrPd within the LRS. The prevalence of infection was influenced by PRNP genetics at codon 142 and by the age of the goats: methionine carriers older than 60 months showed a much lower prevalence of infection (12/78; 15.4%) than those younger than 60 months (20/42; 47.6%); these last showed prevalence values similar to isoleucine homozygotes of any age (40/80; 50.0%). Two of seven goats with definite signs of scrapie were negative for PrPd in brain but positive in LRS tissues, and one goat showed biochemical and IHC features of PrPd different from all other infected goats. The results of this study have implications for surveillance and control policies for scrapie in goats. Revue / Journal Title Veterinary research ISSN 0928-4249 Source / Source 2009, vol. 40, no6, [Note(s): 40:65.1-40:65.13] (34 ref.) Langue / Language Anglais

Editeur / Publisher EDP Sciences, Les Ulis, FRANCE (1993) (Revue)

Mots-clés anglais / English Keywords Farming animal ; Nervous system diseases ; Central nervous system disease ; Degenerative disease ; Cerebral disorder ; Infection ; Prion disease ; Vertebrata ; Mammalia ; Ungulata ; Artiodactyla ; Prion ; Sheep ; Scrapie ; Veterinary ; Microbiology ; Spongiform encephalopathy ; Age ; Genotype ; Epidemiology ; Prevalence ; Goat ; Mots-clés français / French Keywords Animal élevage ; Pathologie du système nerveux ; Pathologie du système nerveux central ; Maladie dégénérative ; Pathologie de l'encéphale ; Infection ; Maladie à prions ; Vertebrata ; Mammalia ; Ungulata ; Artiodactyla ; Association genetique ; Prion ; Mouton ; Tremblante ; Vétérinaire ; Microbiologie ; Encéphalopathie spongiforme ; Age ; Génotype ; Epidémiologie ; Prévalence ; Chèvre ; Mots-clés espagnols / Spanish Keywords Animal cría ; Sistema nervioso patología ; Sistema nervosio central patología ; Enfermedad degenerativa ; Encéfalo patología ; Infección ; Prion enfermedad ; Vertebrata ; Mammalia ; Ungulata ; Artiodactyla ; Prion ; Carnero ; Scrapie ; Veterinario ; Microbiología ; Encefalopatía espongiforme ; Edad ; Genotipo ; Epidemiología ; Prevalencia ; Cabra ; Mots-clés d'auteur / Author Keywords scrapie ; goat ; prion disease ; transmissible spongiform encephalopathy ; Localisation / Location INIST-CNRS, Cote INIST : 14119, 35400017127359.0140

Nº notice refdoc (ud4) : 22076139


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